The District of Columbia Organic Act of 1801 (officially An Act Concerning the District of Columbia) (6th Congress, 2nd Sess., ch. 15, 2 Stat. 103, enacted 1801-02-27) is an Act of Congress, which incorporated the District of Columbia and divided the territory into two counties: Washington County to the east of the Potomac River and Alexandria County to the west. The charters of the independent cities of Georgetown and Alexandria were left in place and no change was made to their status. The laws of both Maryland and Virginia continued to remain in force within the District.

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